Endohm - 適用6mm培養杯(24孔/盤)

(EVOM2跨膜電阻儀專用)

Endohm for 6 mm Culture Cup (24 wells per plate)

型號：ENDOHM-6

個別培養杯中的TEER測量

使用 WPI EVOM2跨膜電阻儀，Endohm可於重複進行細胞培養杯中內皮組織的電阻測量。將培養杯移至Endohm測量，Endohm小室和上蓋各有一對同心電 極：中央的電壓傳感銀/氯化銀小球，加上一個環形電流電極。頂部電極高度可調整以搭配不同製造商的細胞培養杯。Endohm的對稱相對圓盤電極，位於膜上 與膜下，相較於STX2手持式電極讓更為均勻的電流密度流過細胞膜。空白培養杯的背景電阻由150歐姆（使用STX2電極）減少至低於5歐姆。

經濟型內皮歐姆計

Endohm 的固定電極幾何結構，給定樣本的讀數變異由10-30歐姆（使用STX2電極，測量數值依使用者經驗而定)減少至1-2歐姆。相較於其他電阻測量方 式，EVOM2搭配Endohm提供「滲漏性」組織(leaky tissue)更為方便且經濟的解決方案。因為EVOM2的AC矩形波電流的均勻密度，大幅消除了電極極化或膜電容導致的誤差。截至今日，已使用 Costar、Millipore、ICN Biomedicals和Falcon的培養杯進行測試。Endohm可使用EtO、酒精或殺菌劑進行滅菌，不可進行autoclave。

兼容以下chamber：

＊必須正確平衡三點支撐架，使filter與電極平行。

TEER measurement in individual cups

Using WPI’s EVOM2 resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial tissue in culture cups. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups of different manufacture. Endohm’s symmetrically opposing circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density to flow across the membrane than with STX2 electrodes. The background resistance of a blank insert is reduced from 150ohm (when using WPI’s hand-held STX2 electrodes) to less than 5ohm.

Economical endothelial ohmmeter

With Endohm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30 ohm with STX2 electrodes (depending on the experience of the user) to 1-2. Compared with other resistance measurement methods, Endohm with EVOM2 offers a much more convenient and economic solution to “leaky tissue” measurement. Because of the uniform density of the AC square wave current from EVOM2, errors owing to electrode polarization or membrane capacitance are largely eliminated. Endohm together with EVOM offers the most accurate and economical endothelial ohmmeter now avail-able. To date, cups from Costar, Millipore, ICN Biomedicals, and Falcon have been tested. Endohm chambers may be sterilized with EtO, alcohol or a bactericide; not autoclavable.

The ENDOHM-6 is compatible with the following chambers:

* The tri-support legs must be balanced correctly so that the filter is parallel to the electrodes.