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跨膜電阻儀
EVOM2, Epithelial Volt/Ohm Meter for TEER
型號: EVOM2

  • 手動測量TEER在6/12/24孔盤中的上皮細胞。
  • 電氣隔絕,可插入標準插座,無須按鈕即可連續讀取。
  • 可兼容Endohm Chamber。
  • 內含手動操作電極STX2和測試電極。
  • 附傾斜支架,更容易觀察測量結果。
  • 標準類比BNC輸出端口。

 

進行TEER測量以確定細胞全滿 (Cellular Confluence)

EVOM是第一台專門設計用於組織培養研究中,例行性跨上皮電阻 (TEER, Transepithelial Electrical Resistance)測量的裝置。EVOM2為新一代設計,讓操作更為容易。EVOM2不僅可定性測量細胞單層健康,也可定量測量細胞全滿 (Cellular Confluence)。此裝置特有的電極迴路,搭配內附的STX2電極可檢測細胞單層(cellular monolayer);若搭配Endohm chamber,EVOM2可執行更精確的定量測量,或更低電阻的測量,例如:跨上皮電阻測量。

 

EVOM2特色

EVOM2隔離電源是為避免組織不良反應和電極形成金屬沉積而特別設計 (接上插座也可以作用),讓您在需要時隨時可以使用EVOM2。內建充電電池,可使用長達10小時。4.5位讀數可顯示1~9999歐姆;內附測試電極,研究人員可在每次進行電阻測量時校正,以獲得精確讀數;伏特計則不須校正。類比BNC輸出提供資料紀錄或遠程顯示之輸出端口。

 

標準配置:筷狀電極

EVOM2內附廣受歡迎的筷狀電極STX2,寬4mm、厚1mm。電極對上均包含一個銀/氯化銀小球 (用於電壓測量)和一個銀電極 (用於傳輸電流)。小尺寸電極設計,方便搭配各種標準細胞培養杯使用。

膜電壓範圍
+/- 199.0mV
電壓測量  0.1mV
電阻範圍  0-9,999 ohm
交流電矩形波電流 +/- 10uA nominal at 12.5 Hz
電源

內置可充電 6VNiMH 2700mAH 電池,12VDC外接電源充電

額定電池運行時間
8 小時
類比輸出 毫伏模式: 1mV = 10mV
歐姆模式: 1Ω = 1mV
尺寸 19x11x6cm (7.25x4.25x2.30")
重量 1.4kg (3lb)
電極連接 RJ-11 連接器 (電話線類型)
測試電極 1000 ohm 外接
環境範圍 10-38C (50-100F) 
0-90% 無凝結相對濕度
電源 通用 100-240VAC, 120VDC (5.5 x 2.5mm barrel positive tip), 850mA (WPI #800496)

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http://doi.org/10.1172/jci.insight.86355

 

 

Making TEER Measurements to Determine Cellular Confluence

The EVOM was the first instrument designed specifically to perform routine TEER (Transepithelial Electrical Resistance) measurement in tissue culture research. EVOM2 is the next generation, redesigned for ease of use. The EVOM2 not only qualitatively measures cell monolayer health, but also quantitatively measures cellular confluence. The uniqeu electronic circuit of the EVOM2 and the included STX2 electrode detect the confluence of the cellular monolayer. When combined with WPI's Endohm chamber, the EVOM2 can also be used to perform more accurate quantitative measurement or lower resistance measurements like trans endothelial electrical resistance measurements.

 

Features of the EVOM2

The isolated power source of the EVOM2 was specifically designed to avoid adverse effects on tissue and the formation of electrode metal deposits, even when it is plugged into a standard wall outlet. Now, the EVOM2 is always on when you need it. In addition, its rechargeable battery allows up to 10 hours of mobile use. The four and a half digit readout provides a range of 1-9,999 ohms. The included test electrode lets you calibrate the resistance measurements for an accurate reading every time, and the voltage meter never needs calibration. An analog BNC output is standard with the EVOM2, providing an output port for recording data or remote display of the EVOM2 output. 

 

Chopstick Electrodes Included with the EVOM2

EVOM2 comes complete with the popular STX2 "chopstick" electrodes, 4mm wide and 1mm thick. Each stick of the electrode pair contains a silver/silver-chloride pellet for measuring voltage and a silver electrode for passing current. The small size of each electrode is designed to facilitate placement of the electrodes into a variety of standard cell culture wells.


Specification

 

 

 
 
 
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