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Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Cblb: Casitas B-lineage lymphoma b
Origin
The transgenic rats were established by injection of transgene consisting of wild-type Cblb into Sprague Dawley (Slc:SD) rat fertile eggs in 2000, followed by backcrossing the transgenic rats onto KDP rats. This transgenic rat expresses the wild-type cDNA of Cblb gene under the control of mouse H2-Kd promoter. (09.7.9)
Strain characteristics
KDP is a spontaneous animal model of human type 1 diabetes and a nonsense mutation in Cblb was identified as a causative gene. To confirm that the Cblb mutation is responsible for the phenotype of the KDP rat, wild-type Cblb gene was introduced to KDP rats as rescue experiments. As a result, this transgenic strain was rescued partially (about 30% diabetes incidence as show in KDP) (Yokoi, 2002). (09.7.9)
Breeding Conditions
Homozygous Cblb rats are usually infertile in both sexes and are therefore maintained by crossing heterozygous individuals (segregating inbred strain).
Genotyping
References
Yokoi N, Komeda K, Wang HY, Yano H, Kitada K, Saitoh Y, Seino Y, Yasuda K, Serikawa T, Seino S.
Cblb is a major susceptibility gene for rat type 1 diabetes mellitus.
Nat Genet. 31(4):391-4, 2002
Yokoi N, Namae M, Fuse M, Wang HY, Hirata T, Seino S, Komeda K.
Establishment and characterization of the Komeda diabetes-prone rat as a segregating inbred strain.
Exp Anim. 52(4):295-301, 2003
Embryo 
Sperm 