跨膜電阻儀
EVOM2, Epithelial Volt/Ohm Meter for TEER
型號： EVOM2

• 手動測量TEER在6/12/24孔盤中的上皮細胞。
  
• 電氣隔絕，可插入標準插座，無須按鈕即可連續讀取。
  
• 可兼容Endohm Chamber。
• 內含手動操作電極STX2和測試電極。
  
• 附傾斜支架，更容易觀察測量結果。
• 標準類比BNC輸出端口。

進行TEER測量以確定細胞全滿 (Cellular Confluence)

EVOM是第一台專門設計用於組織培養研究中，例行性跨上皮電阻 (TEER, Transepithelial Electrical Resistance)測量的裝置。EVOM2為新一代設計，讓操作更為容易。EVOM2不僅可定性測量細胞單層健康，也可定量測量細胞全滿 (Cellular Confluence)。此裝置特有的電極迴路，搭配內附的STX2電極可檢測細胞單層(cellular monolayer)；若搭配Endohm chamber，EVOM2可執行更精確的定量測量，或更低電阻的測量，例如：跨上皮電阻測量。

EVOM2特色

EVOM2隔離電源是為避免組織不良反應和電極形成金屬沉積而特別設計 (接上插座也可以作用)，讓您在需要時隨時可以使用EVOM2。內建充電電池，可使用長達10小時。4.5位讀數可顯示1~9999歐姆；內附測試電極，研究人員可在每次進行電阻測量時校正，以獲得精確讀數；伏特計則不須校正。類比BNC輸出提供資料紀錄或遠程顯示之輸出端口。

標準配置：筷狀電極

EVOM2內附廣受歡迎的筷狀電極STX2，寬4mm、厚1mm。電極對上均包含一個銀/氯化銀小球 (用於電壓測量)和一個銀電極 (用於傳輸電流)。小尺寸電極設計，方便搭配各種標準細胞培養杯使用。

Reference

Di, S., Gujie, M., & Thomas, W. (2016). Magnetic ferri-liposomes for triggered drug release across the blood- brain barrier. Frontiers in Bioengineering and Biotechnology, 4. http://doi.org/10.3389/conf.FBIOE.2016.01.00061 

Paradis, A., Leblanc, D., & Dumais, N. (2016). Optimization of an in vitro human blood-brain barrier model: Application to blood monocyte transmigration assays. MethodsX, 3, 25–34. http://doi.org/10.1016/j.mex.2015.11.009 

Meenach, S. A., Tsoras, A. N., McGarry, R. C., Mansour, H. M., Hilt, J. Z., & Anderson, K. W. (2016). Development of three-dimensional lung multicellular spheroids in air- and liquid-interface culture for the evaluation of anticancer therapeutics. International Journal of Oncology, 48(4), 1701–9. http://doi.org/10.3892/ijo.2016.3376 

Turdalieva, A., Solandt, J., Shambetova, N., Xu, H., Blom, H., Brismar, H., … Fu, Y. (2016). Bioelectric and Morphological Response of Liquid-Covered Human Airway Epithelial Calu-3 Cell Monolayer to Periodic Deposition of Colloidal 3-Mercaptopropionic-Acid Coated CdSe-CdS/ZnS Core-Multishell Quantum Dots. PloS One, 11(2), e0149915. http://doi.org/10.1371/journal.pone.0149915 

Tosoni, K., Cassidy, D., Kerr, B., Land, S. C., & Mehta, A. (2016). Using Drugs to Probe the Variability of Trans-Epithelial Airway Resistance. PloS One, 11(2), e0149550. http://doi.org/10.1371/journal.pone.0149550 

Iacovelli, J., Rowe, G. C., Khadka, A., Diaz-Aguilar, D., Spencer, C., Arany, Z., & Saint-Geniez, M. (2016). PGC-1α Induces Human RPE Oxidative Metabolism and Antioxidant Capacity. Investigative Ophthalmology & Visual Science, 57(3), 1038–51. http://doi.org/10.1167/iovs.15-17758 

Melvin, J. A., Lashua, L. P., Kiedrowski, M. R., Yang, G., Deslouches, B., Montelaro, R. C., & Bomberger, J. M. (2016). Simultaneous Antibiofilm and Antiviral Activities of an Engineered Antimicrobial Peptide during Virus-Bacterium Coinfection. mSphere, 1(3). http://doi.org/10.1128/mSphere.00083-16 

Bartakova, A., Alvarez-Delfin, K., Weisman, A. D., Salero, E., Raffa, G. A., Merkhofer, R. M., … Goldberg, J. L. (2016). Novel Identity and Functional Markers for Human Corneal Endothelial Cells. Investigative Ophthalmology & Visual Science, 57(6), 2749–62. http://doi.org/10.1167/iovs.15-18826 

Mansley, M. K., Watt, G. B., Francis, S. L., Walker, D. J., Land, S. C., Bailey, M. A., & Wilson, S. M. (2016). Dexamethasone and insulin activate serum and glucocorticoid-inducible kinase 1 (SGK1) via different molecular mechanisms in cortical collecting duct cells. Physiological Reports, 4(10). http://doi.org/10.14814/phy2.12792 

Griffin, J. M., Kho, D., Graham, E. S., Nicholson, L. F. B., & O’Carroll, S. J. (2016). Statins Inhibit Fibrillary β-Amyloid Induced Inflammation in a Model of the Human Blood Brain Barrier. PloS One, 11(6), e0157483. http://doi.org/10.1371/journal.pone.0157483 

Bernocchi, B., Carpentier, R., Lantier, I., Ducournau, C., Dimier-Poisson, I., & Betbeder, D. (2016). Mechanisms allowing protein delivery in nasal mucosa using NPL nanoparticles. Journal of Controlled Release : Official Journal of the Controlled Release Society, 232, 42–50. http://doi.org/10.1016/j.jconrel.2016.04.014 

Yan, Y., Shapiro, A. P., Mopidevi, B. R., Chaudhry, M. A., Maxwell, K., Haller, S. T., … Liu, J. (2016). Protein Carbonylation of an Amino Acid Residue of the Na/K-ATPase α1 Subunit Determines Na/K-ATPase Signaling and Sodium Transport in Renal Proximal Tubular Cells. Journal of the American Heart Association, 5(9). http://doi.org/10.1161/JAHA.116.003675 

Gu, M. J., Song, S. K., Lee, I. K., Ko, S., Han, S. E., Bae, S., … Yun, C.-H. (2016). Barrier protection via Toll-like receptor 2 signaling in porcine intestinal epithelial cells damaged by deoxynivalnol. Veterinary Research, 47, 25. http://doi.org/10.1186/s13567-016-0309-1 

Ahmed, C. M., Biswal, M. R., Li, H., Han, P., Ildefonso, C. J., & Lewin, A. S. (2016). Repurposing an orally available drug for the treatment of geographic atrophy. Molecular Vision, 22, 294–310. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/27110092 

Tsata, V., Velegraki, A., Ioannidis, A., Poulopoulou, C., Bagos, P., Magana, M., & Chatzipanagiotou, S. (2016). Effects of Yeast and Bacterial Commensals and Pathogens of the Female Genital Tract on the Transepithelial Electrical Resistance of HeLa Cells. The Open Microbiology Journal, 10, 90–6. http://doi.org/10.2174/1874285801610010090 

Lewis, S. B., Prior, A., Ellis, S. J., Cook, V., Chan, S. S. M., Gelson, W., & Schüller, S. (2016). Flagellin Induces β-Defensin 2 in Human Colonic Ex vivo Infection with Enterohemorrhagic Escherichia coli. Frontiers in Cellular and Infection Microbiology, 6, 68. http://doi.org/10.3389/fcimb.2016.00068 

Gallagher, E., Minn, I., Chambers, J. E., & Searson, P. C. (2016). In vitro characterization of pralidoxime transport and acetylcholinesterase reactivation across MDCK cells and stem cell-derived human brain microvascular endothelial cells (BC1-hBMECs). Fluids and Barriers of the CNS, 13(1), 10. http://doi.org/10.1186/s12987-016-0035-0 

Williams, K. M., Gokulan, K., Cerniglia, C. E., & Khare, S. (2016). Size and dose dependent effects of silver nanoparticle exposure on intestinal permeability in an in vitro model of the human gut epithelium. Journal of Nanobiotechnology, 14(1), 62. http://doi.org/10.1186/s12951-016-0214-9 

Torr, E., Heath, M., Mee, M., Shaw, D., Sharp, T. V, & Sayers, I. (2016). Expression of polycomb protein BMI-1 maintains the plasticity of basal bronchial epithelial cells. Physiological Reports, 4(16). http://doi.org/10.14814/phy2.12847 

Slater, M., Torr, E., Harrison, T., Forrester, D., Knox, A., Shaw, D., & Sayers, I. (2016). The differential effects of azithromycin on the airway epithelium in vitro and in vivo. Physiological Reports, 4(18). http://doi.org/10.14814/phy2.12960 

Miao, W., Wu, X., Wang, K., Wang, W., Wang, Y., Li, Z., … Peng, L. (2016). Sodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2. International Journal of Molecular Sciences, 17(10). http://doi.org/10.3390/ijms17101696 

Fiandra, L., Mazzucchelli, S., Truffi, M., Bellini, M., Sorrentino, L., & Corsi, F. (2016). <em>In Vitro</em> Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules. Journal of Visualized Experiments, (114), e54279–e54279. http://doi.org/10.3791/54279 

Saeedi, B. J., Kao, D. J., Kitzenberg, D. A., Dobrinskikh, E., Schwisow, K. D., Masterson, J. C., … Glover, L. E. (2015). HIF-dependent regulation of claudin-1 is central to intestinal epithelial tight junction integrity. Molecular Biology of the Cell, 26(12), 2252–62. http://doi.org/10.1091/mbc.E14-07-1194 

Lin, B., Liu, Y., Li, T., Zeng, K., Cai, S., Zeng, Z., … Gao, Y. (2015). Ulinastatin mediates protection against vascular hyperpermeability following hemorrhagic shock. International Journal of Clinical and Experimental Pathology, 8(7), 7685–93. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/26339335 

Datta, P., & Weis, M. T. (2015). Calcium glycerophosphate preserves transepithelial integrity in the Caco-2 model of intestinal transport. World Journal of Gastroenterology, 21(30), 9055–66. http://doi.org/10.3748/wjg.v21.i30.9055 

Travanty, E., Zhou, B., Zhang, H., Di, Y. P., Alcorn, J. F., Wentworth, D. E., … Wang, J. (2015). Differential Susceptibilities of Human Lung Primary Cells to H1N1 Influenza Viruses. Journal of Virology, 89(23), 11935–44. http://doi.org/10.1128/JVI.01792-15 

Brown, J. A., Pensabene, V., Markov, D. A., Allwardt, V., Neely, M. D., Shi, M., … Wikswo, J. P. (2015). Recreating blood-brain barrier physiology and structure on chip: A novel neurovascular microfluidic bioreactor. Biomicrofluidics, 9(5), 54124. http://doi.org/10.1063/1.4934713 

Deosarkar, S. P., Prabhakarpandian, B., Wang, B., Sheffield, J. B., Krynska, B., & Kiani, M. F. (2015). A Novel Dynamic Neonatal Blood-Brain Barrier on a Chip. PloS One, 10(11), e0142725. http://doi.org/10.1371/journal.pone.0142725 

Mladenova, K., Petrova, S., Moskova-Doumanova, V., Topouzova-Hristova, T., Stoitsova, S., Tabashka, I., … Doumanov, J. (2015). Transepithelial resistance in human bestrophin-1 stably transfected Madin-Darby canine kidney cells. Biotechnology, Biotechnological Equipment, 29(1), 101–104. http://doi.org/10.1080/13102818.2014.988078 

Qosa, H., Batarseh, Y. S., Mohyeldin, M. M., El Sayed, K. A., Keller, J. N., & Kaddoumi, A. (2015). Oleocanthal Enhances Amyloid-β Clearance from the Brains of TgSwDI Mice and in Vitro across a Human Blood-Brain Barrier Model. ACS Chemical Neuroscience, 6(11), 1849–1859. http://doi.org/10.1021/acschemneuro.5b00190 

Ma, W., Feng, S., Yao, X., Yuan, Z., Liu, L., & Xie, Y. (2015). Nobiletin enhances the efficacy of chemotherapeutic agents in ABCB1 overexpression cancer cells. Scientific Reports, 5, 18789. http://doi.org/10.1038/srep18789 

Khan, N., Pantakani, D. V. K., Binder, L., Qasim, M., & Asif, A. R. (2015). Immunosuppressant MPA Modulates Tight Junction through Epigenetic Activation of MLCK/MLC-2 Pathway via p38MAPK. Frontiers in Physiology, 6, 381. http://doi.org/10.3389/fphys.2015.00381 

Schexnayder, C., & Stratford, R. E. (2015). Genistein and Glyceollin Effects on ABCC2 (MRP2) and ABCG2 (BCRP) in Caco-2 Cells. International Journal of Environmental Research and Public Health, 13(1), ijerph13010017. http://doi.org/10.3390/ijerph13010017 

Marvin, S. A., Huerta, C. T., Sharp, B., Freiden, P., Cline, T. D., & Schultz-Cherry, S. (2015). Type I Interferon Response Limits Astrovirus Replication and Protects against Increased Barrier Permeability In Vitro and In Vivo. Journal of Virology, 90(4), 1988–96. http://doi.org/10.1128/JVI.02367-15 

Curtis, V. F., Ehrentraut, S. F., Campbell, E. L., Glover, L. E., Bayless, A., Kelly, C. J., … Colgan, S. P. (2015). Stabilization of HIF through inhibition of Cullin-2 neddylation is protective in mucosal inflammatory responses. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 29(1), 208–15. http://doi.org/10.1096/fj.14-259663 

Loma, P., Guzman-Aranguez, A., Pérez de Lara, M. J., & Pintor, J. (2015). Diadenosine tetraphosphate induces tight junction disassembly thus increasing corneal epithelial permeability. British Journal of Pharmacology, 172(4), 1045–58. http://doi.org/10.1111/bph.12972 

Xu, Q., Liu, J., Wang, Z., Guo, X., Zhou, G., Liu, Y., … Su, L. (2015). Heat stress-induced disruption of endothelial barrier function is via PAR1 signaling and suppressed by Xuebijing injection. PloS One, 10(2), e0118057. http://doi.org/10.1371/journal.pone.0118057 

Lever, A. R., Park, H., Mulhern, T. J., Jackson, G. R., Comolli, J. C., Borenstein, J. T., … Prantil-Baun, R. (2015). Comprehensive evaluation of poly(I:C) induced inflammatory response in an airway epithelial model. Physiological Reports, 3(4). http://doi.org/10.14814/phy2.12334 

Biswal, M. R., Ahmed, C. M., Ildefonso, C. J., Han, P., Li, H., Jivanji, H., … Lewin, A. S. (2015). Systemic treatment with a 5HT1a agonist induces anti-oxidant protection and preserves the retina from mitochondrial oxidative stress. Experimental Eye Research, 140, 94–105. http://doi.org/10.1016/j.exer.2015.07.022 

Chen, S., Einspanier, R., & Schoen, J. (2015). Transepithelial electrical resistance (TEER): a functional parameter to monitor the quality of oviduct epithelial cells cultured on filter supports. Histochemistry and Cell Biology, 144(5), 509–15. http://doi.org/10.1007/s00418-015-1351-1 

Prewitt, A. R., Ghose, S., Frump, A. L., Datta, A., Austin, E. D., Kenworthy, A. K., & de Caestecker, M. P. (2015). Heterozygous null bone morphogenetic protein receptor type 2 mutations promote SRC kinase-dependent caveolar trafficking defects and endothelial dysfunction in pulmonary arterial hypertension. The Journal of Biological Chemistry, 290(2), 960–71. http://doi.org/10.1074/jbc.M114.591057 

Lewis, S. B., Cook, V., Tighe, R., & Schüller, S. (2015). Enterohemorrhagic Escherichia coli colonization of human colonic epithelium in vitro and ex vivo. Infection and Immunity, 83(3), 942–9. http://doi.org/10.1128/IAI.02928-14 

Leir, S.-H., Browne, J. A., Eggener, S. E., & Harris, A. (2015). Characterization of primary cultures of adult human epididymis epithelial cells. Fertility and Sterility, 103(3), 647–54.e1. http://doi.org/10.1016/j.fertnstert.2014.11.022 

Laksitorini, M. D., Kiptoo, P. K., On, N. H., Thliveris, J. A., Miller, D. W., & Siahaan, T. J. (2015). Modulation of intercellular junctions by cyclic-ADT peptides as a method to reversibly increase blood-brain barrier permeability. Journal of Pharmaceutical Sciences, 104(3), 1065–75. http://doi.org/10.1002/jps.24309 

Liu, Z., Zhang, F., Koh, G. Y., Dong, X., Hollingsworth, J., Zhang, J., … Stout, R. W. (2015). Cytotoxic and antiangiogenic paclitaxel solubilized and permeation-enhanced by natural product nanoparticles. Anti-Cancer Drugs, 26(2), 167–79. http://doi.org/10.1097/CAD.0000000000000173 

Tian, K. Y., Liu, X. J., Xu, J. D., Deng, L. J., & Wang, G. (2015). Propofol inhibits burn injury-induced hyperpermeability through an apoptotic signal pathway in microvascular endothelial cells. Brazilian Journal of Medical and Biological Research = Revista Brasileira de Pesquisas Medicas E Biologicas, 48(5), 401–7. http://doi.org/10.1590/1414-431X20144107 

Roos, S., Wyder, M., Candi, A., Regenscheit, N., Nathues, C., van Immerseel, F., & Posthaus, H. (2015). Binding studies on isolated porcine small intestinal mucosa and in vitro toxicity studies reveal lack of effect of C. perfringens beta-toxin on the porcine intestinal epithelium. Toxins, 7(4), 1235–52. http://doi.org/10.3390/toxins7041235

Eady, J. J., Wormstone, Y. M., Heaton, S. J., Hilhorst, B., & Elliott, R. M. (2015). Differential effects of basolateral and apical iron supply on iron transport in Caco-2 cells. Genes & Nutrition, 10(3), 463. http://doi.org/10.1007/s12263-015-0463-5 

Venter, J., Francis, H., Meng, F., DeMorrow, S., Kennedy, L., Standeford, H., … Alpini, G. (2015). Development and functional characterization of extrahepatic cholangiocyte lines from normal rats. Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 47(11), 964–72. http://doi.org/10.1016/j.dld.2015.07.012 

Park, S. W., Kim, J. H., Park, S. M., Moon, M., Lee, K. H., Park, K. H., … Kim, J. H. (2015). RAGE mediated intracellular Aβ uptake contributes to the breakdown of tight junction in retinal pigment epithelium. Oncotarget, 6(34), 35263–73. http://doi.org/10.18632/oncotarget.5894 

Erami, Z., Timpson, P., Yao, W., Zaidel-Bar, R., & Anderson, K. I. (2015). There are four dynamically and functionally distinct populations of E-cadherin in cell junctions. Biology Open, 4(11), 1481–9. http://doi.org/10.1242/bio.014159 

Zaccone, E. J., Goldsmith, W. T., Shimko, M. J., Wells, J. R., Schwegler-Berry, D., Willard, P. A., … Fedan, J. S. (2015). Diacetyl and 2,3-pentanedione exposure of human cultured airway epithelial cells: Ion transport effects and metabolism of butter flavoring agents. Toxicology and Applied Pharmacology, 289(3), 542–9. http://doi.org/10.1016/j.taap.2015.10.004 

Le, M. T., van Veldhuizen, M., Porcelli, I., Bongaerts, R. J., Gaskin, D. J. H., Pearson, B. M., & van Vliet, A. H. M. (2015). Conservation of σ28-Dependent Non-Coding RNA Paralogs and Predicted σ54-Dependent Targets in Thermophilic Campylobacter Species. PloS One, 10(10), e0141627. http://doi.org/10.1371/journal.pone.0141627 

Ross, B. N., Rojas-Lopez, M., Cieza, R. J., McWilliams, B. D., & Torres, A. G. (2015). The Role of Long Polar Fimbriae in Escherichia coli O104:H4 Adhesion and Colonization. PloS One, 10(10), e0141845. http://doi.org/10.1371/journal.pone.0141845 

Blenkinsop, T. A., Saini, J. S., Maminishkis, A., Bharti, K., Wan, Q., Banzon, T., … Stern, J. H. (2015). Human Adult Retinal Pigment Epithelial Stem Cell-Derived RPE Monolayers Exhibit Key Physiological Characteristics of Native Tissue. Investigative Ophthalmology & Visual Science, 56(12), 7085–99. http://doi.org/10.1167/iovs.14-16246 

Baddal, B., Muzzi, A., Censini, S., Calogero, R. A., Torricelli, G., Guidotti, S., … Pezzicoli, A. (2015). Dual RNA-seq of Nontypeable Haemophilus influenzae and Host Cell Transcriptomes Reveals Novel Insights into Host-Pathogen Cross Talk. mBio, 6(6), e01765-15. http://doi.org/10.1128/mBio.01765-15 

Patella, F., Schug, Z. T., Persi, E., Neilson, L. J., Erami, Z., Avanzato, D., … Zanivan, S. (2015). Proteomics-based metabolic modeling reveals that fatty acid oxidation (FAO) controls endothelial cell (EC) permeability. Molecular & Cellular Proteomics : MCP, 14(3), 621–34. http://doi.org/10.1074/mcp.M114.045575 

Mansourpour, M., Mahjub, R., Amini, M., Ostad, S. N., Shamsa, E. S., Rafiee-Tehrani, M., & Dorkoosh, F. A. (2015). Development of acid-resistant alginate/trimethyl chitosan nanoparticles containing cationic β-cyclodextrin polymers for insulin oral delivery. AAPS PharmSciTech, 16(4), 952–62. http://doi.org/10.1208/s12249-014-0282-9 

Xing, F., Sharma, S., Liu, Y., Mo, Y.-Y., Wu, K., Zhang, Y.-Y., … Watabe, K. (2015). miR-509 suppresses brain metastasis of breast cancer cells by modulating RhoC and TNF-α. Oncogene, 34(37), 4890–900. http://doi.org/10.1038/onc.2014.412 

Cieza, R. J., Hu, J., Ross, B. N., Sbrana, E., & Torres, A. G. (2015). The IbeA invasin of adherent-invasive Escherichia coli mediates interaction with intestinal epithelia and macrophages. Infection and Immunity, 83(5), 1904–18. http://doi.org/10.1128/IAI.03003-14 

Hind, W. H., Tufarelli, C., Neophytou, M., Anderson, S. I., England, T. J., & O’Sullivan, S. E. (2015). Endocannabinoids modulate human blood-brain barrier permeability in vitro. British Journal of Pharmacology, 172(12), 3015–27. http://doi.org/10.1111/bph.13106 

Ferguson, M. C., Saul, S., Fragkoudis, R., Weisheit, S., Cox, J., Patabendige, A., … Fazakerley, J. K. (2015). Ability of the Encephalitic Arbovirus Semliki Forest Virus To Cross the Blood-Brain Barrier Is Determined by the Charge of the E2 Glycoprotein. Journal of Virology, 89(15), 7536–49. http://doi.org/10.1128/JVI.03645-14 

Kuehn, D., Majeed, S., Guedj, E., Dulize, R., Baumer, K., Iskandar, A., … Peitsch, M. C. (2015). Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke. Journal of Visualized Experiments, (96), e52325–e52325. http://doi.org/10.3791/52325 

Cao, X., Lin, H., Muskhelishvili, L., Latendresse, J., Richter, P., Heflich, R. H., … Browning, M. (2015). Tight junction disruption by cadmium in an in vitro human airway tissue model, 16(1), 30. http://doi.org/10.1186/s12931-015-0191-9 

Srinivasan, B., Kolli, A. R., Esch, M. B., Abaci, H. E., Shuler, M. L., & Hickman, J. J. (2015). TEER measurement techniques for in vitro barrier model systems., 20(2). http://doi.org/10.1177/2211068214561025 

Pothoven, K. L., Norton, J. E., Hulse, K. E., Suh, L. A., Carter, R. G., Rocci, E., … Schleimer, R. P. (2015). Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. The Journal of Allergy and Clinical Immunology, 136(3), 737–746.e4. http://doi.org/10.1016/j.jaci.2015.01.043 

Chraïbi, A., & Renauld, S. (2014). PPARγ-induced stimulation of amiloride-sensitive sodium current in renal collecting duct principal cells is serum and insulin dependent. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 33(3), 581–93. http://doi.org/10.1159/000358636 

Schneditz, G., Rentner, J., Roier, S., Pletz, J., Herzog, K. A. T., Bücker, R., … Zechner, E. L. (2014). Enterotoxicity of a nonribosomal peptide causes antibiotic-associated colitis. Proceedings of the National Academy of Sciences of the United States of America, 111(36), 13181–6. http://doi.org/10.1073/pnas.1403274111 

Molenda, N., Urbanova, K., Weiser, N., Kusche-Vihrog, K., Günzel, D., Schillers, H., … Howell, S. (2014). Paracellular Transport through Healthy and Cystic Fibrosis Bronchial Epithelial Cell Lines – Do We Have a Proper Model? PLoS ONE, 9(6), e100621. http://doi.org/10.1371/journal.pone.0100621 

Grover, A., Hirani, A., Pathak, Y., & Sutariya, V. (2014). Brain-targeted delivery of docetaxel by glutathione-coated nanoparticles for brain cancer. AAPS PharmSciTech, 15(6), 1562–8. http://doi.org/10.1208/s12249-014-0165-0 

Guzman-Aranguez, A., Calvo, P., Ropero, I., & Pintor, J. (2014). In vitro effects of preserved and unpreserved anti-allergic drugs on human corneal epithelial cells. Journal of Ocular Pharmacology and Therapeutics : The Official Journal of the Association for Ocular Pharmacology and Therapeutics, 30(9), 790–8. http://doi.org/10.1089/jop.2014.0030 

Sjöqvist, S., Jungebluth, P., Lim, M. L., Haag, J. C., Gustafsson, Y., Lemon, G., … Macchiarini, P. (2014). Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats. Nature Communications, 5, 3562. http://doi.org/10.1038/ncomms4562 

Wise, S. K., Laury, A. M., Katz, E. H., Den Beste, K. A., Parkos, C. A., & Nusrat, A. (2014). Interleukin-4 and interleukin-13 compromise the sinonasal epithelial barrier and perturb intercellular junction protein expression. International Forum of Allergy & Rhinology, 4(5), 361–70. http://doi.org/10.1002/alr.21298 

Czupalla, C. J., Liebner, S., & Devraj, K. (2014). In Vitro Models of the Blood–Brain Barrier (pp. 415–437). http://doi.org/10.1007/978-1-4939-0320-7_34 

Czupalla, C. J., Liebner, S., & Devraj, K. (2014). In vitro models of the blood-brain barrier. Methods in Molecular Biology (Clifton, N.J.), 1135, 415–37. http://doi.org/10.1007/978-1-4939-0320-7_34 

Roh-Johnson, M., Bravo-Cordero, J. J., Patsialou, A., Sharma, V. P., Guo, P., Liu, H., … Condeelis, J. (2014). Macrophage contact induces RhoA GTPase signaling to trigger tumor cell intravasation. Oncogene, 33(33), 4203–12. http://doi.org/10.1038/onc.2013.377 

De Chiara, L., Fagoonee, S., Ranghino, A., Bruno, S., Camussi, G., Tolosano, E., … Altruda, F. (2014). Renal cells from spermatogonial germline stem cells protect against kidney injury. Journal of the American Society of Nephrology : JASN, 25(2), 316–28. http://doi.org/10.1681/ASN.2013040367 

McIntyre, B. A. S., Alev, C., Mechael, R., Salci, K. R., Lee, J. B., Fiebig-Comyn, A., … Bhatia, M. (2014). Expansive generation of functional airway epithelium from human embryonic stem cells. Stem Cells Translational Medicine, 3(1), 7–17. http://doi.org/10.5966/sctm.2013-0119 

Bohara, M., Kambe, Y., Nagayama, T., Tokimura, H., Arita, K., & Miyata, A. (2014). C-type natriuretic peptide modulates permeability of the blood-brain barrier. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism, 34(4), 589–96. http://doi.org/10.1038/jcbfm.2013.234 

Yu, J., Li, N., Lin, P., Li, Y., Mao, X., Bao, G., … Zhao, R. (2014). Intestinal transportations of main chemical compositions of polygoni multiflori radix in caco-2 cell model. Evidence-Based Complementary and Alternative Medicine : eCAM, 2014, 483641. http://doi.org/10.1155/2014/483641 

Van Itallie, C. M., Tietgens, A. J., Aponte, A., Fredriksson, K., Fanning, A. S., Gucek, M., & Anderson, J. M. (2014). Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion. Journal of Cell Science, 127(Pt 4), 885–95. http://doi.org/10.1242/jcs.140475 

Wang, L., Luo, H., Chen, X., Jiang, Y., & Huang, Q. (2014). Functional characterization of S100A8 and S100A9 in altering monolayer permeability of human umbilical endothelial cells. PloS One, 9(3), e90472. http://doi.org/10.1371/journal.pone.0090472 

McHugh, K. J., Tao, S. L., & Saint-Geniez, M. (2014). Porous poly(ε-caprolactone) scaffolds for retinal pigment epithelium transplantation. Investigative Ophthalmology & Visual Science, 55(3), 1754–62. http://doi.org/10.1167/iovs.13-12833 

Al-Ghoul, W. M., Kim, M. S., Fazal, N., Azim, A. C., & Ali, A. (2014). Evidence for simvastatin anti-inflammatory actions based on quantitative analyses of NETosis and other inflammation/oxidation markers. Results in Immunology, 4, 14–22. http://doi.org/10.1016/j.rinim.2014.03.001

Keenan, C. R., Mok, J. S., Harris, T., Xia, Y., Salem, S., & Stewart, A. G. (2014). Bronchial epithelial cells are rendered insensitive to glucocorticoid transactivation by transforming growth factor-β1. Respiratory Research, 15(1), 55. http://doi.org/10.1186/1465-9921-15-55 

Uehara, I., Kimura, T., Tanigaki, S., Fukutomi, T., Sakai, K., Shinohara, Y., … Sakurai, H. (2014). Paracellular route is the major urate transport pathway across the blood-placental barrier. Physiological Reports, 2(5). http://doi.org/10.14814/phy2.12013 

Enjoji, S., Ohama, T., & Sato, K. (2014). Regulation of epithelial cell tight junctions by protease-activated receptor 2. The Journal of Veterinary Medical Science, 76(9), 1225–9. http://doi.org/10.1292/jvms.14-0191 

Gipson, I. K., Spurr-Michaud, S., Tisdale, A., & Menon, B. B. (2014). Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function. PloS One, 9(6), e100393. http://doi.org/10.1371/journal.pone.0100393 

Couturier, J., Hutchison, A. T., Medina, M. A., Gingaras, C., Urvil, P., Yu, X., … Lewis, D. E. (2014). HIV replication in conjunction with granzyme B production by CCR5+ memory CD4 T cells: Implications for bystander cell and tissue pathologies. Virology, 462–463, 175–88. http://doi.org/10.1016/j.virol.2014.06.008 

Li, Y., Wu, W.-H., Hsu, C.-W., Nguyen, H. V, Tsai, Y.-T., Chan, L., … Tsang, S. H. (2014). Gene therapy in patient-specific stem cell lines and a preclinical model of retinitis pigmentosa with membrane frizzled-related protein defects. Molecular Therapy : The Journal of the American Society of Gene Therapy, 22(9), 1688–97. http://doi.org/10.1038/mt.2014.100 

Sun, H., Harris, W. T., Kortyka, S., Kotha, K., Ostmann, A. J., Rezayat, A., … Clancy, J. P. (2014). Tgf-beta downregulation of distinct chloride channels in cystic fibrosis-affected epithelia. PloS One, 9(9), e106842. http://doi.org/10.1371/journal.pone.0106842 

Li, G., Li, T., Li, Y., Cai, S., Zhang, Z., Zeng, Z., … Chen, Z. (2014). Ulinastatin inhibits oxidant-induced endothelial hyperpermeability and apoptotic signaling. International Journal of Clinical and Experimental Pathology, 7(11), 7342–50. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/25550770 

Costello, C. M., Hongpeng, J., Shaffiey, S., Yu, J., Jain, N. K., Hackam, D., & March, J. C. (2014). Synthetic small intestinal scaffolds for improved studies of intestinal differentiation. Biotechnology and Bioengineering, 111(6), 1222–32. http://doi.org/10.1002/bit.25180 

Meredith, M. E., Qu, Z.-C., & May, J. M. (2014). Ascorbate reverses high glucose- and RAGE-induced leak of the endothelial permeability barrier. Biochemical and Biophysical Research Communications, 445(1), 30–5. http://doi.org/10.1016/j.bbrc.2014.01.078 

Reaves, D. K., Fagan-Solis, K. D., Dunphy, K., Oliver, S. D., Scott, D. W., & Fleming, J. M. (2014). The role of lipolysis stimulated lipoprotein receptor in breast cancer and directing breast cancer cell behavior. PloS One, 9(3), e91747. http://doi.org/10.1371/journal.pone.0091747 

Chimezie, C., Ewing, A. C., Quadri, S. S., Cole, R. B., Boué, S. M., Omari, C. F., … Jr. (2014). Glyceollin transport, metabolism, and effects on p-glycoprotein function in Caco-2 cells. Journal of Medicinal Food, 17(4), 462–71. http://doi.org/10.1089/jmf.2013.0115 

Lei, Y., Stamer, W. D., Wu, J., & Sun, X. (2014). Cell senescence reduced the mechanotransduction sensitivity of porcine angular aqueous plexus cells to elevation of pressure. Investigative Ophthalmology & Visual Science, 55(4), 2324–8. http://doi.org/10.1167/iovs.13-13317 

Nakadate, H., Inuzuka, K., Akanuma, S., Kakuta, A., & Aomura, S. (2014). Effect of amplitude and duration of impulsive pressure on endothelial permeability in in vitro fluid percussion trauma. Biomedical Engineering Online, 13, 44. http://doi.org/10.1186/1475-925X-13-44 

Costello, C. M., Sorna, R. M., Goh, Y.-L., Cengic, I., Jain, N. K., & March, J. C. (2014). 3-D intestinal scaffolds for evaluating the therapeutic potential of probiotics. Molecular Pharmaceutics, 11(7), 2030–9. http://doi.org/10.1021/mp5001422 

Crane, J. K., Broome, J. E., Reddinger, R. M., & Werth, B. B. (2014). Zinc protects against Shiga-toxigenic Escherichia coli by acting on host tissues as well as on bacteria. BMC Microbiology, 14, 145. http://doi.org/10.1186/1471-2180-14-145 

Mishra, R., & Singh, S. K. (2014). HIV-1 Tat C phosphorylates VE-cadherin complex and increases human brain microvascular endothelial cell permeability. BMC Neuroscience, 15, 80. http://doi.org/10.1186/1471-2202-15-80 

Shimko, M. J., Zaccone, E. J., Thompson, J. A., Schwegler-Berry, D., Kashon, M. L., & Fedan, J. S. (2014). Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiological Reports, 2(7). http://doi.org/10.14814/phy2.12073 

Pongkorpsakol, P., Pathomthongtaweechai, N., Srimanote, P., Soodvilai, S., Chatsudthipong, V., & Muanprasat, C. (2014). Inhibition of cAMP-activated intestinal chloride secretion by diclofenac: cellular mechanism and potential application in cholera. PLoS Neglected Tropical Diseases, 8(9), e3119. http://doi.org/10.1371/journal.pntd.0003119 

Arredondo Zamarripa, D., Díaz-Lezama, N., Meléndez García, R., Chávez Balderas, J., Adán, N., Ledesma-Colunga, M. G., … Thebault, S. (2014). Vasoinhibins regulate the inner and outer blood-retinal barrier and limit retinal oxidative stress. Frontiers in Cellular Neuroscience, 8, 333. http://doi.org/10.3389/fncel.2014.00333 

Fossum, S. L., Mutolo, M. J., Yang, R., Dang, H., O’Neal, W. K., Knowles, M. R., … Harris, A. (2014). Ets homologous factor regulates pathways controlling response to injury in airway epithelial cells. Nucleic Acids Research, 42(22), 13588–98. http://doi.org/10.1093/nar/gku1146 

Meenach, S. A., Anderson, K. W., Hilt, J. Z., McGarry, R. C., Mansour, H. M., Samantha A. Meenach, Kimberly W. Anderson, J. Zach Hilt, Ronald C. McGarry, H. M. M., … Mansour, H. M. (2014, December 20). High-Performing Dry Powder Inhalers of Paclitaxel DPPC/DPPG Lung Surfactant-Mimic Multifunctional Particles in Lung Cancer: Physicochemical Characterization, In Vitro Aerosol Dispersion, and Cellular Studies. http://doi.org/10.1208/s12249-014-0182-z 

Ghaffarian, R., & Muro, S. (2013). Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers. Journal of Visualized Experiments, (80), e50638–e50638. http://doi.org/10.3791/50638 

Ao, M., Sarathy, J., Domingue, J., Alrefai, W. A., & Rao, M. C. (2013). Chenodeoxycholic acid stimulates Cl(-) secretion via cAMP signaling and increases cystic fibrosis transmembrane conductance regulator phosphorylation in T84 cells. American Journal of Physiology. Cell Physiology, 305(4), C447-56. http://doi.org/10.1152/ajpcell.00416.2012 

Lei, Y., Stamer, W. D., Wu, J., & Sun, X. (2013). Oxidative stress impact on barrier function of porcine angular aqueous plexus cell monolayers. Investigative Ophthalmology & Visual Science, 54(7), 4827–35. http://doi.org/10.1167/iovs.12-11435 

Chen, L., Zhu, J., Li, Y., Lu, J., Gao, L., Xu, H., … Yang, X. (2013). Enhanced nasal mucosal delivery and immunogenicity of anti-caries DNA vaccine through incorporation of anionic liposomes in chitosan/DNA complexes. PloS One, 8(8), e71953. http://doi.org/10.1371/journal.pone.0071953 

Schmedt, T., Chen, Y., Nguyen, T. T., Li, S., Bonanno, J. A., Jurkunas, U. V., … Giasson, C. (2012). Telomerase Immortalization of Human Corneal Endothelial Cells Yields Functional Hexagonal Monolayers. PLoS ONE, 7(12), e51427. http://doi.org/10.1371/journal.pone.0051427 

Guzman-Aranguez, A., Woodward, A. M., Pintor, J., & Argüeso, P. (2012). Targeted disruption of core 1 β1,3-galactosyltransferase (C1galt1) induces apical endocytic trafficking in human corneal keratinocytes. PloS One, 7(5), e36628. http://doi.org/10.1371/journal.pone.0036628 

Alhamoruni, A., Lee, A. C., Wright, K. L., Larvin, M., & O’Sullivan, S. E. (2010). Pharmacological Effects of Cannabinoids on the Caco-2 Cell Culture Model of Intestinal Permeability. Journal of Pharmacology and Experimental Therapeutics, 335(1).

Campbell, L., Abulrob, A.-N. G., Kandalaft, L. E., Plummer, S., Hollins, A. J., Gibbs, A., & Gumbleton, M. (2003). Constitutive Expression of P-Glycoprotein in Normal Lung Alveolar Epithelium and Functionality in Primary Alveolar Epithelial Cultures. Journal of Pharmacology and Experimental Therapeutics, 304(1).

S. Jayaraman, Y. Song, L.Vetrivel, L.Shankar, A. S. V. (2001). Noninvasive in vivo fluorescence measurement of airway-surface liquid depth, salt concentration, and pH. The Journal of Clinical Investigation, 107(3), 317. http://doi.org/10.1172/JCI11154 

Teoh, D. A., Kamieniecki, D., Pang, G., & Buret, A. G. (2000). GIARDIA LAMBLIA REARRANGES F-ACTIN AND α-ACTININ IN HUMAN COLONIC AND DUODENAL MONOLAYERS AND REDUCES TRANSEPITHELIAL ELECTRICAL RESISTANCE. Journal of Parasitology, 86(4), 800. http://doi.org/10.1645/0022-3395(2000)086[0800:GLRFAA]2.0.CO;2 

Meerveld, G.-V. B., R, T. K., 文 タ イ ト ル和, & vitro の ラ ッ ト 空 腸 お よ び 結 腸 に お け る 木 ク レ オ ソ － ト お よ び ロ ペ ラ ミ ド の 止 瀉 効 果 の 比 較, I. (2000). Comparison of the antidiarrheal effects of wood creosote and loperamide in the rat jejunum and colon in vitro. Biol Pharm Bull, 23, 952–956.

Blaisdell, C. J., Edmonds, R. D., Wang, X. T., Guggino, S., & Zeitlin, P. L. (2000). pH-regulated chloride secretion in fetal lung epithelia. American Journal of Physiology. Lung Cellular and Molecular Physiology, 278(6), L1248-55. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10835331 

Yang, J. J., Kim, K. J., & Lee, V. H. (2000). Role of P-glycoprotein in restricting propranolol transport in cultured rabbit conjunctival epithelial cell layers. Pharmaceutical Research, 17(5), 533–8. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10888304 

Utsumi, H., Chiba, H., Kamimura, Y., Osanai, M., Igarashi, Y., Tobioka, H., … Sawada, N. (2000). Expression of GFRα-1, receptor for GDNF, in rat brain capillary during postnatal development of the BBB. American Journal of Physiology - Cell Physiology, 279(2).

Raimondi, F., Kao, J. P., Fiorentini, C., Fabbri, A., Donelli, G., Gasparini, N., … Fasano, A. (2000). Enterotoxicity and cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin in in vitro systems. Infection and Immunity, 68(6), 3180–5. http://doi.org/10.1128/IAI.68.6.3180-3185.2000 

Reichert, M., Müller, T., & Hunziker, W. (2000). The PDZ domains of zonula occludens-1 induce an epithelial to mesenchymal transition of Madin-Darby canine kidney I cells. Evidence for a role of beta-catenin/Tcf/Lef signaling. The Journal of Biological Chemistry, 275(13), 9492–500. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10734097 

Chang, J.-E., Basu, S. K., & Lee, V. H. L. (2000). Air-Interface Condition Promotes the Formation of Tight Corneal Epithelial Cell Layers for Drug Transport Studies. Pharmaceutical Research, 17(6), 670–676. http://doi.org/10.1023/A:1007569929765 

Hilgendorf, C., Spahn‐Langguth, H., Regårdh, C. G., Lipka, E., Amidon, G. L., & Langguth, P. (2000). Caco‐2 versus Caco‐2/HT29‐MTX Co‐cultured Cell Lines: Permeabilities Via Diffusion, Inside‐ and Outside‐Directed Carrier‐Mediated Transport. Journal of Pharmaceutical Sciences, 89(1), 63–75. http://doi.org/10.1002/(SICI)1520-6017(200001)89:1<63::AID-JPS7>3.0.CO;2-6 

Johnson, L. G., Olsen, J. C., Naldini, L., & Boucher, R. C. (2000). Pseudotyped human lentiviral vector-mediated gene transfer to airway epithelia in vivo. Gene Therapy, 7(7), 568–574. http://doi.org/10.1038/sj.gt.3301138 

Bu, HZ; Poglod, M; Micetich, RG; Khan, J. (2000). High-throughput Caco-2 cell permeability screening by cassette dosing and sample pooling approaches using direct injection/on-line guard cartridge extraction/tandem mass spectrometry. Retrieved November 15, 2016, from http://serials.unibo.it/cgi-ser/start/en/spogli/df-s.tcl?prog_art=7030068&language=ENGLISH&view=articoli 

Castro, R., Barlow-Walden, L., Woodson, T., Kerecman, J. D., Zhang, G. H., & Martinez, J. R. (2000). Ion transport in an immortalized rat submandibular cell line SMG-C6. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 225(1), 39–48. http://doi.org/10.1046/J.1525-1373.2000.22505.X 

Takakuwa, R., Kokai, Y., Kojima, T., Akatsuka, T., Tobioka, H., Sawada, N., & Mori, M. (2000). Uncoupling of Gate and Fence Functions of MDCK Cells by the Actin-Depolymerizing Reagent Mycalolide B. Experimental Cell Research, 257(2), 238–244. http://doi.org/10.1006/excr.2000.4887 

Garate, M. A., & Nunez, M. T. (2000). Overexpression of the Ferritin Iron-responsive Element Decreases the Labile Iron Pool and Abolishes the Regulation of Iron Absorption by Intestinal Epithelial (Caco-2) Cells. Journal of Biological Chemistry, 275(3), 1651–1655. http://doi.org/10.1074/jbc.275.3.1651 

Katz, J., Sambandam, V., Wu, J. H., Michalek, S. M., & Balkovetz, D. F. (2000). Characterization of Porphyromonas gingivalis-induced degradation of epithelial cell junctional complexes. Infection and Immunity, 68(3), 1441–9. http://doi.org/10.1128/IAI.68.3.1441-1449.2000 

Busch, C., Hofmann, F., Gerhard, R., & Aktories, K. (2000). Involvement of a conserved tryptophan residue in the UDP-glucose binding of large clostridial cytotoxin glycosyltransferases. The Journal of Biological Chemistry, 275(18), 13228–34. http://doi.org/10.1074/JBC.275.18.13228 

Wang, G., Zabner, J., Deering, C., Launspach, J., Shao, J., Bodner, M., … McCray, P. B. (2000). Increasing Epithelial Junction Permeability Enhances Gene Transfer to Airway Epithelia In Vivo. American Journal of Respiratory Cell and Molecular Biology, 22(2), 129–138. http://doi.org/10.1165/ajrcmb.22.2.3938 

Robledo, R. F., Barber, D. S., & Witten, M. L. (1999). Modulation of bronchial epithelial cell barrier function by in vitro jet propulsion fuel 8 exposure. Toxicological Sciences : An Official Journal of the Society of Toxicology, 51(1), 119–25. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10496683 

Liu, D. Z., Morris-Natschke, S. L., Kucera, L. S., Ishaq, K. S., Thakker, D. R., Hoogdalem, E. J. Van, … Magnusson, C. (1999). Structure-activity relationships for enhancement of paracellular permeability by 2-alkoxy-3-alkylamidopropylphosphocholines across Caco-2 cell monolayers. Journal of Pharmaceutical Sciences, 88(11), 1169–74. http://doi.org/10.1021/JS9900957 

Liu, D.-Z., Lecluyse, E. L., & Thakker, D. R. (1999). Dodecylphosphocholine-mediated enhancement of paracellular permeability and cytotoxicity in Caco-2 cell monolayers. Journal of Pharmaceutical Sciences, 88(11), 1161–1168. http://doi.org/10.1021/js990094e 

Francis, S. A., Kelly, J. M., McCormack, J., Rogers, R. A., Lai, J., Schneeberger, E. E., & Lynch, R. D. (1999). Rapid reduction of MDCK cell cholesterol by methyl-beta-cyclodextrin alters steady state transepithelial electrical resistance. European Journal of Cell Biology, 78(7), 473–84. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10472800 

Ali, M. H., Schlidt, S. A., Chandel, N. S., Hynes, K. L., Schumacker, P. T., & Gewertz, B. L. (1999). Endothelial permeability and IL-6 production during hypoxia: role of ROS in signal transduction. The American Journal of Physiology, 277(5 Pt 1), L1057-65. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10564193 

Igarashi, Y., Utsumi, H., Chiba, H., Yamada-Sasamori, Y., Tobioka, H., Kamimura, Y., … Sawada, N. (1999). Glial cell line-derived neurotrophic factor induces barrier function of endothelial cells forming the blood-brain barrier. Biochemical and Biophysical Research Communications, 261(1), 108–12. http://doi.org/10.1006/bbrc.1999.0992 

Planchon, S., Fiocchi, C., Takafuji, V., & Roche, J. K. (1999). Transforming growth factor-?1 preserves epithelial barrier function: Identification of receptors, biochemical intermediates, and cytokine antagonists. Journal of Cellular Physiology, 181(1), 55–66. http://doi.org/10.1002/(SICI)1097-4652(199910)181:1<55::AID-JCP6>3.0.CO;2-M 

Defoe, D. M., & Easterling, K. C. (1994). Reattachment of retinas to cultured pigment epithelial monolayers from Xenopus laevis. Investigative Ophthalmology & Visual Science, 35(5), 2466–2476.

Dickman, K. G., Hempson, S. J., Anderson, J., Lippe, S., Zhao, L., Burakoff, R., & Shaw, R. D. (n.d.). Rotavirus alters paracellular permeability and energy metabolism in Caco-2 cells.

Sawai, T., Usui, N., Dwaihy, J., Drongowski, R. A., Abe, A., Coran, A. G., & Harmon, C. M. (n.d.). The effect of phospholipase A2 on bacterial translocation in a cell culture model. Pediatric Surgery International, 16(4), 262–266. http://doi.org/10.1007/S003830050741 

Davis, B. P., Stucke, E. M., Khorki, M. E., Litosh, V. A., Rymer, J. K., Rochman, M., … Orlando, R. (2016). Eosinophilic esophagitis–linked calpain 14 is an IL-13–induced protease that mediates esophageal epithelial barrier impairment. JCI Insight, 1(4), 895–900.

http://doi.org/10.1172/jci.insight.86355

Making TEER Measurements to Determine Cellular Confluence

The EVOM was the first instrument designed specifically to perform routine TEER (Transepithelial Electrical Resistance) measurement in tissue culture research. EVOM2 is the next generation, redesigned for ease of use. The EVOM2 not only qualitatively measures cell monolayer health, but also quantitatively measures cellular confluence. The uniqeu electronic circuit of the EVOM2 and the included STX2 electrode detect the confluence of the cellular monolayer. When combined with WPI's Endohm chamber, the EVOM2 can also be used to perform more accurate quantitative measurement or lower resistance measurements like trans endothelial electrical resistance measurements.

Features of the EVOM2

The isolated power source of the EVOM2 was specifically designed to avoid adverse effects on tissue and the formation of electrode metal deposits, even when it is plugged into a standard wall outlet. Now, the EVOM2 is always on when you need it. In addition, its rechargeable battery allows up to 10 hours of mobile use. The four and a half digit readout provides a range of 1-9,999 ohms. The included test electrode lets you calibrate the resistance measurements for an accurate reading every time, and the voltage meter never needs calibration. An analog BNC output is standard with the EVOM2, providing an output port for recording data or remote display of the EVOM2 output. 

Chopstick Electrodes Included with the EVOM2

EVOM2 comes complete with the popular STX2 "chopstick" electrodes, 4mm wide and 1mm thick. Each stick of the electrode pair contains a silver/silver-chloride pellet for measuring voltage and a silver electrode for passing current. The small size of each electrode is designed to facilitate placement of the electrodes into a variety of standard cell culture wells.

Specification